Phil Jagielka pulls out of squad with injury
Phil Jagielka has pulled out of the England squad to face Malta and Slovenia.
The 34-year-old Everton defender picked up an injury in training, the Football Association have announced.
England host Malta on Saturday (17:00 BST) and visit Slovenia on Tuesday (19:45 BST) in World Cup qualifiers.
Keane, 23, was added to the squad after Glen Johnson also withdrew through injury.
Southgate called up winger Andros Townsend on Monday as a replacement for the injured Raheem Sterling.
But he has no immediate plans to call up a replacement for Jagielka.
The Everton skipper has played in every league game this season under Ronald Koeman and has looked reinvigorated under the Dutch coach.
Goalkeepers: Fraser Forster (Southampton), Joe Hart (Torino, on loan from Manchester City), Tom Heaton (Burnley)
Defenders: Ryan Bertrand (Southampton), Gary Cahill (Chelsea), Michael Keane (Burnley), Danny Rose (Tottenham), Chris Smalling (Manchester United), John Stones (Manchester City), Kyle Walker (Tottenham)
Midfielders: Eric Dier (Tottenham), Jordan Henderson (Liverpool), Alex Oxlade-Chamberlain (Arsenal), Michail Antonio (West Ham), Jesse Lingard (Manchester United), Theo Walcott (Arsenal), Dele Alli (Tottenham), Andros Townsend (Crystal Palace)
Strikers: Wayne Rooney (Manchester United), Marcus Rashford (Manchester United), Daniel Sturridge (Liverpool), Jamie Vardy (Leicester)